Internal Validation ISO 17025

 

We provide services for validation of your specific qPCR kits. Our validation report will contribute to increase the quality of your internal assay procedure, and will support evaluation during full method validation and/or accreditations to international norms.

Parameters for validation of qPCR kits with intend to meet the standards of UNE-EN ISO/IEC 17025 and AOAC International guidelines.

  • Specificity (inclusivity and exclusivity) by using bacterial type strains from reference culture collections.
  • Validation of standard calibration curve, analysis of variance and efficiency (accuracy and precision).
  • Determination of limits for detection and quantification.
  • Inhibitory effect of sample.
  • Reliability characteristics: repeatability, reproducibility, and measurement uncertainty.

 

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Research of Veterinary Diseases