One-Day Food Testing
Detection of pathogens in a single day
GPS™ has developed a protocol for foodborne pathogen control that allows to advance the results in a single working day. Time of each step has been reduced and the procedure has been simplified and optimized. Easy, safe and fast.
Rapid extraction (<20 min) of DNA from samples enriched in Peptone Broth and Fraser media
- Elimination of PCR inhibitors
- Validated in different matrices:
vegetables, meat, dairy, cereals and fruits
Each PCR tube contains all the reagents needed to perform the detection test for each specific pathogen. Just add the sample!
GPS™ has optimized its reagents to use a thermo-cycling protocol with very short reaction times (8 min). 
* qPCR total time depends on your thermocycler ramp rate
“Anticipate the quality in IV Range food”
Internal GPS™ validation of qPCR kits according to international standard UNE-EN ISO/IEC 17025
Terms of validation
The validations have been performed following the guidelines of the international standard UNE-EN ISO 17025. Several aspects of the tests were based on the standards UNE-EN ISO 6579 (Salmonella), UNE-EN ISO 11290-1 (Listeria Monocytogenes) and UNE-EN ISO 16654 (Escherichia coli O157:H7). For questions related to PCR, UNE-EN ISO 22118 was reviewed.
Specifity of qPCR:
-Inclusivity / Exclusivity (in silico & in vitro).
Quantitative PCR phase (calibration and statistical analysis n≥10):
-Principle of validation of the standard curve (106 to 10 copies).
-Protocol of evaluation of the standard curve.
-Estimation of linear regression (R2).
-Validation of the linear model.
-Validation of efficiency (PCR performance).
Reliability of the analysis:
-Repeatability.
-Reproducibility.
Sensitivity of the method:
-Limit of detection (LOD).
-Limit of quantification (LOQ).
All parameters were tested for n= 10 experiments
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