MONODOSE dtec-qPCR Test

 

 

 

“New format, dehydrated and ready-to-use”

 

This innovative product are individual ready-to-use tubes containing all the components needed for detection of specific pathogens: dNTPs, polymerase, pathogen-specific primers and probe, and the appropriate buffer. 

Monodose dtec qPCR Test

MONODOSE advantages

 

Dispensing aliquots for PCR from a master solution, with addition of primer/probe, has some disadvantages: rupture of the enzyme by freezing/thawing, risks of cross-contamination, fluorophore deterioration by UV light and time consuming. Some benefits are listed below:

 

  • Just add your diluted sample to each tube and run qPCR.
  • As reactions are dehydrated, can be transported at room temperature.
  • Optimized MONODOSE dtec-qPCR Tests shows no difference compared to the non-dehydrated ordinary qPCR.
  • Our HotStart Taq polymerase is very stable in absence of water and is rapidly activated with only 1 minute at 95 ºC.
  • Thermal regime is quick and the same for all our kits, you may perform detection of different pathogens in the same run.
  • Compatibility with all qPCR devices, plate based and glass capillary. 

           Take the MONODOSE tubes you need to test from the fridge, no need defrosting, no risk of contamination...

...Just add sample and run PCR with our standard protocol.

OPERATION

TEMP.

TIME

CYCLES

Activation

95ºC

60 secs

1

Denaturation

95ºC

10 secs

40

Annealing/Elongation

60ºC

60 sec 

 

 

 

 MONODOSE PRICES

 DNA Target
·M24 (24 DOSE + Standard)   148€
·M96 (96 DOSE + Standard)   398€
  RNA Target
·M60 (60 DOSE + Standard)     322€
·M120 (120 DOSE + Standard) 584€

 

                                                                                       

Technical Review

 Fig. 3. Calibration curve of the MTplex qPCR-test by dispensing ordinary mix (Grey) or by using the MONODOSE Dtec-qPCR at first day of preparation (red) day 0; 2nd day (yellow); day 4 (green); and after 7 days (blue).  Assayed points showed no dispersion  

104 copies

102 copies

Our HotStart Taq polymerase is very stable in absence of water and is rapidly activated in only 1 minute at 95ºC.

MONODOSE Dtec-qPCR optimised to give the best performance and faster.

Fig. 1. MTplex qPCR-test by dispensing ordinary mix (grey) and, curves obtained with Monodose qPCR-Test (red)

106 copies


104 copies

Experiments performed with MONODOSE Dtec-qPCR demonstrated that is stable at least up to 7 days at room temperature. (Fig.2. sensitivity and intensity remains).

102 copies

MONODOSE Dtec-qPCR may be transported at room temperature reducing shipping and disposal costs but also making our product friendly to the environment.

Fig. 2. Kinetics obtained from the MTplex qPCR-test by dispensing ordinary mix (Grey) or by using the MONODOSE Dtec-qPCR at day of preparation (red) day 0; 2nd day (yellow); day 4 ( green); and after 7 days (blue). 


Reproducibility was assessed after 2, 4, and 7 days maintained

at room Tª. As showed in Figure 3, assayed points 102, 104, and

106 (genomic copies), showed no dispersion

Fig. 3. Calibration curve of the MTplex qPCR-test by dispensing ordinary mix (Grey) or by using the MONODOSE Dtec-qPCR at first day of preparation (red) day 0; 2nd day (yellow); day 4 ( green); and after 7 days (blue).  Assayed points showed no dispersion.