TargetSpecies dtec-qPCR Test 

 

           “We build, validate, and package, qPCR tests for any specific pathogen in less than 2 weeks”

 list of available kits 

Competitive format with all the components included for the best qPCR assay!

Product features                                                                                                                                                                   

    Kits PCR Real Time

                                                                                   

  • Includes a mastermix stable at room temperature.
  • Thermal regime is quick and the same for all our kits.
  • Updated primer specificity by phylogenetic analysis.
  • Each batch is validated and a calibration curve is provided.
  • Standard template with known copy number provided for quantification curve.
  • High priming efficiency and broad dynamic detection range (>6 logs).
  • Sensitive to 5 copies of target.

DNA TargetSpecies dtec-qPCR content:

TargetSpecies dtec-qPCR-mix (primers and probe)

Resuspension buffer

Standard template for calibration curve (2x107 copies)

RNAse/DNAse free ultrapure water

Mastermix MixStable qPCR.5X:

Price for 100 reactions, 318 €

RNA TargetSpecies dtec-RT-qPCR content:

TargetSpecies dtec RT-qPCR-mix (primers and probe)

Resuspension buffer

Standard template for calibration curve (2x107 copies)

RNAse/DNAse free ultrapure water

Mastermix LyoMix RT-qPCR (One-Step):

Price for 100 reactions, 388 €

 *GPS primers and probes are sold for research use only.

Target specificity for microbial detection

The sequence specificity of gen targets is crucial in the design of primers for species-specific detection. The target sequence should be conserved for all strains of a single species (inclusivity) but also should show enough nucleotide variation or mismatches to these homologous sequences from all the other, related or not, species and genera (exclusivity). Above attributes highly relative as they depend of:

  • the species: not all species evolve at the same rate
  • evolving nature of the sequences: not all show the same evolution mode, conservation
  • and more difficult, the diversity/data available at public databases

Consequently, very often, most available primers and probes published and available in commercial kits fail to detect some strains of the target species, or give false positives in the presence of phylogenetically related taxa. GPS™ expertise in microbial evolution provides robust frames for this purposes. Our team uses phylogenetic criteria to select the best possible target for species-specificity guarantied.

Sequence design of primers and probes Universally known and requested primer/probe attributes are under control by personal dedication of our experts and using the best software available for design of qPCR primers and probes. An optimal design for real time PCR includes the following characteristics:

  • Minimize primer dimers
  • Minimize primer hairpins
  • Optimal Tm
  • Optimal G+C content
  • 3'-end GC clamp
  • Short amplicons
  • No template secondary structure
  • Probe melting point
  • Probe length
  • Probe G/C content

Validation of your pathogen detection qPCR Kits

We provide services for validation of your specific qPCR kits. Our validation report will contribute to increase the quality of your internal assay procedure, and will support evaluation during full method validation and/or accreditations to international norms.

The parameters for validation of qPCR kits intend to meet the standards of UNE-EN ISO/IEC 17025 and AOAC International guidelines. The specificity (inclusivity and exclusivity) was checked by using bacterial type strains from reference culture collections. Validation of standard calibration curve, analysis of variance and efficiency (accuracy and precision). Determination of limits of detection and quantification; inhibitory effect of real samples. Reliability characteristics: repeatabilityreproducibility, and measurement uncertainty.

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