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All qPCR kits provided by GPS are validated at batch production. GPS showed a vast experience designing procedures that may be adapted to follow guidelines of the international norm UNE-EN ISO/IEC 17025

GPS provide to customers with technical support during starting-up of the RT-PCR systems implemented in the laboratories. Specific support is also given by manufacturers represented by GPS. Further questions while running routine tests will also be attended by our expertise technicians.

GPS counts with the collaboration of a group of experts on taxonomy and genetics from the University Miguel Hernandez leadership by Dr A. Martínez-Murcia, a member of several prestigious national and international committees.


Dr Antonio Martínez-Murcia
Director & Business Development

Dr Antonio Martinez Murcia

 

Curriculum 

Dr. Antonio Martínez-Murcia (B.Sc. Biochemist/Molecular Biologist, Valencia University 1987; Ph.D. Microbiology, Reading University, UK, 1993), professor of microbiology (Molecular Pathogenicity and Vaccines) at the University “Miguel Hernandez”, Orihuela, Alicante, where also was promoter and director of the “Unidad de Diagnóstico Molecular” (2001). He counts with 30 years’ experience in genetic analysis applied to bacterial phylogeny, evolution, taxonomy, typing, identification and molecular microbial ecology. He has participated in > 30 national and international financed projects. He is co-author of ca. 80 papers in peer-reviewed journals and book chapters, a patented PCR method (1994), co-director of 8 doctoral theses, and has described 15 new bacterial species. He is an active member of the “Aeromonas Working-group” of the “International Sub-Committee on Vibrionaceae, Aeromonadaceae, and related genera” (International Committee on Systematics of Prokariotes). He is the promoter and director of GENETIC ANALYSIS STRATEGIES SL, developing PCR assays for > 250 different pathogens of interest in veterinary, food, water, and clinical environment. Several biotech entrepreneur activities and innovations were awarded in 2002, 2012, and 2015.

Publications

 1. A phylogenetic analysis of the genus Leuconostoc based on reverse transcriptase sequencing of 16S rRNA. Martínez-Murcia, A.J . and Collins, M.D. 1990. FEMS Microbiol. Lett., 70: 73-84.

2. 16S ribosomal ribonucleic acid sequence determination of members of the genus Carnobacterium and related lactic acid bacteria. Description of Vagococcus salmoninarum sp. nov. Wallbanks, S., Martínez-Murcia, A.J., Fryer, J.L., Phillips, B.A. and Collins M.D. 1990. Int. J. System. Bacteriol., 40: 224-230.

3. Phylogenetic analysis of the genus Lactobacillus and related lactic acid bacteria as determined by reverse transcriptase of 16S rRNA. Collins, M.D., Rodrigues, U., Ash, C., Aguirre, M., Farrow, J.A.E., Martínez-Murcia, A.J., Phillips, B.A., Williams, A.M. and Wallbanks, S. 1990. FEMS Microbiol. Lett., 77: 5-12.

4. Nucleotide sequence of 16S rRNA from Lactobacillus kandleri and Lactobacillus minor. Martínez-Murcia, A.J. and Collins, M.D. 1990. Nuc. Acids Res., 18: 3401.

5. Nucleotide sequence of 16S rRNA from Lactobacillus confusus and Lactobacillus viridescens. Martínez-Murcia, A.J. and Collins, M.D. 1990. Nuc. Acids Res., 18: 3402.

6. Enterococcus sulfureus, a new yellow-pigmented enterococci species. Martínez-Murcia, A.J. and Collins, M.D. 1991. FEMS Microbiol. Lett., 80: 69-73.

7. A phylogenetic analysis of an atypical leuconostoc: description of Leuconostoc fallax sp. nov. Martínez-Murcia, A.J. and Collins, M.D. 1991. FEMS Microbiol. Lett., 82: 55-60.

8. An automated RNA extraction procedure and application for 16S rRNA sequencing of Leuconostoc amelobiosum. Martínez-Murcia, A.J. 1991. Microbiología SEM, 7: 106-112.

9. Phylogenetic interrelationships of members of the genera Aeromonas and Plesiomonas as determined by 16S rDNA sequencing: lack of congruence with results of DNA-DNA hybridizations. Martínez-Murcia, A.J., Benlloch, S. and Collins, M.D. 1992. Int. J. Sys. Bacteriol., 42 : 412-421.

10. Aeromonas allosaccharophila sp. nov., a new mesophilic member of the genus Aeromonas. Martínez-Murcia, A.J., Esteve, C., Garay, E. and Collins, M.D. 1992. FEMS Microbiol. Lett., 91, 199-206.

11. Phylogenetic analysis of some leuconostocs and related organisms as determined from large-subunit rRNA gene sequences: assessment of congruence of small- and large-subunit rRNA derived trees. Martinez-Murcia, A.J., Harland, N.M. and Collins, M.D. 1993. J. Appl. Bacteriol., 74, 532-541.

12. Identification of Aeromonas schubertii and Aeromonas jandaei by using a polymerase chain reaction-probe test. Martinez-Murcia, A.J. and Collins, M.D. 1993. FEMS Microbiol. Lett., 108, 151-156.

13. Molecular identification of Aeromonas sobria by polymerase chain reaction. Ash, C., Martínez-Murcia, A.J. and Collins, M.D. 1993. Med. Microbiol. Lett., 2, 80-86.

14. Aeromonas enteropelogenes and Aeromonas ichthiosmia are identical to Aeromonas trota and Aeromonas veronii, respectively, as revealed by small-subunit rRNA sequence analysis. Collins, M.D., Martínez-Murcia, A.J. and Cai, J. 1993. Int. J. Sys. Bacteriol., 43, 855-866.

15. Random amplified polymorphic DNA of a group of halophilic archaeal isolates. Martínez-Murcia, A.J. and Rodríguez-Valera, F. 1994. System. Appl. Microbiol., 17, 395-401.

16.The use of arbitrarily primed PCR (AP-PCR) to develop taxa specific DNA probes of known sequence. Martínez-Murcia, A.J. and Rodríguez-Valera, F. 1994. FEMS Microbiol. Lett., 124, 265-270.

17. Evaluation of the authenticity of haloarchaeal strains by Random-amplified polymorphic DNA. Martínez-Murcia, A.J., Boan, I.J. and Rodríguez-Valera, F. 1995. Lett. Appl. Microbiol., 21, 106-108.

18. Sequencing of Bacterial and Archaeal 16S rRNA genes directly amplified from a hypersalineenvironment. Benlloch, S., Martínez-Murcia, A.J. and Rodríguez-Valera,F. 1995. Syst. Appl. Microbiol., 18, 574-581.

19. Evaluation of prokaryotic diversity by restrictase digestion of 16S rDNA directly amplified from hypersaline environments. Martínez-Murcia A.J., Acinas, S.G. and Rodríguez-Valera, F. 1995. FEMS Microbiol. Ecol., 17, 247-256.

20. Bacterial diversity in two coastal lagoons deduced from 16S rDNA PCR amplification and partial sequencing. Benlloch, S., Rodríguez-Valera,F and Martínez-Murcia, A.J. 1995. FEMS Microbiol. Ecol., 18, 267-280

21. Molecular evidence supporting the existence of two major groups in uropathogenic Escherichia coli. García-Martínez, J., Martínez-Murcia, A.J., Rodríguez-Valera, F. and Zorraquino, A. 1996. FEMS Immunol. Med. Microbiol., 14, 231-244.

22.16S-23S rDNA intergenic sequences indicate that Leuconostoc oenos is phylogenetically homogeneous. Zavaleta, A., Martínez-Murcia, A.J. and Rodríguez-Valera,F. 1996. Microbiology-UK, 142, 2105-2114.

23. Genetic diversity in uropathogenic Escherichia coli as shown by RAPD (Random Amplified Polymorphic DNA), 16S-23S rDNA spacers and adhesin gene restriction patterns. In Book: “Ecology of pathogenic bacteria: molecular and evolutionary aspects”. BAM van der Zeijst, WPM Hoekstra, JDA van Embden and AJW Alphen Editors. Royal Netherlands Academy of Arts and Sciences, Amsterdam, The Netherlands. Rodríguez-Valera, F., García-Martínez, J., Zorraquino, A. and Martínez-Murcia, A.J. 1997.

24. Intraspecific diversity in/strongspan bacteria. The case of Escherichia coli . Rodríguez-Valera, F. García-Martínez and Martínez-Murcia, A.J. 1995. Microbiología SEM, 11, 379-382.

25. Heterotrophic bacteria activity and bacterial diversity in two coastal lagoons as detected by culture and 16S rDNA PCR amplification and partial sequencing. Benlloch, S., Rodríguez-Valera,F., Acinas, S.G. and Martínez-Murcia, A.J. 1996. Hydrobiology 329, 3-17.

26.Description of prokaryotic biodiversity along the salinity gradient of a multipond solar saltern by direct PCR amplification of 16S rDNA. Benlloch, S., Acinas, S.G., Martínez-Murcia, A.J. and Rodríguez-Valera,F. 1996. Hydrobiology 329, 19-31. 

27. Heterotrophic bacteria activity and bacterial diversity in two coastal lagoons as detected by culture and 16S rDNA PCR amplification and partial sequencing. Benlloch, S., Rodríguez-Valera,F., Acinas, S.G. and Martínez-Murcia, A.J. 1996. Developments in Hydrobiology. “Coastal Lagoon Eutrophication and ANaerobic Processes (C.L.E.A.N.)”. Pierre Caumette, Jacques Castel and Rodney Herbert, Editors. Kluwer Academic Publishers, Dordrecht, The Netherlands.

28. Description of prokaryotic biodiversity along the salinity gradient of a multipond solar saltern by direct PCR amplification of 16S rDNA. Benlloch, S., Acinas, S.G., Martínez-Murcia, A.J. and Rodríguez-Valera,F. 1996. Developments in Hydrobiology. “Coastal Lagoon Eutrophication and ANaerobic Processes (C.L.E.A.N.)”. Pierre Caumette, Jacques Castel and Rodney Herbert, Editors. Kluwer Academic Publishers, Dordrecht, The Netherlands.

29. Patterns and sequencing analyses of DNA-fragments randomly amplified from Leuconostoc oenos. Zavaleta, A., Martínez-Murcia, A.J. and Rodríguez-Valera, F. 1997 Appl. Environ. Microbiol. 63, 1261-1267

30. Comparison of the small 16S to 23S intergenic spacer region (ISR) of the rRNA operons of some Escherichia coli of the ECOR collection and E. coli K12. García-Martínez, J., Martínez-Murcia, A.J., Anton, A.I., and Rodríguez-Valera,F. 1996. J. of Bacteriol. 178, 6374-6377.

31. Identification of Aeromonas clinical isolates by PCR-amplified 16S rDNA-restriction fragment length polymorphism. Borrell, N., Acinas, S.G., Figueras, M.J. and Martínez-Murcia, A.J. 1997. J. Clin. Microbiol. 35, 1671-1674.

32. Patterns of sequence variation in two regions of the 16S rRNA multigene family of Escherichia coli.. Martinez-Murcia, A.J., Anton, A.I. and Rodríguez-Valera, F. 1999. Int.J. Sys. Bacteriol.49, 601-610.

33. Intergenic spacer region (ISR) of the rRNA operons in representatives of the Escherichia coli ECOR collection. Antón, A.I., Martinez-Murcia,A.J. and Rodríguez-Valera, F. 1998. J. Mol. Evol. 47, 62-72.

34. Intraspecific diversity of the 23S rRNA gene and the spacer region downstream in Escherichia coli. Antón, A.I., Martinez-Murcia,A.J. and Rodríguez-Valera, F. 1999 J. Bacteriol. 181, 2703-2709.

35. Phylogenetic positions of Aeromonas encheleia, Aeromonas popoffii, Aeromonas DNA Hybridization Group 11 and Aeromonas Group 501. Martinez-Murcia,A.J. 1999. Int. J. Syst. Bacteriol. 49, 1403-1408.

36. Typing of clinical and environmental Aeromonas veronii strains based on the 16S-23S rDNA spacers. Martinez-Murcia,A.J., Borrell, N. and Figueras, M.-J. 2000. FEMS Inmunol. Med. Microbiol. Lett. 28, 225-232.

37. Extended method for discrimination of Aeromonas spp. By 16S rDNA RFLP analysis. Figueras, M.J., Soler, L., Chacón, M., Guarro, J. and Martínez-Murcia, A.J. 2000 Int. J. Sys. Evol. Microbiol. 50, 2069-2073.

38. Clynically relevant Aeromonas spp. Figueras, M.J., Guarro, J. and Martínez-Murcia, A.J. 2000 Clin. Infect. Dis. 30, 988-989

39. Restriction fragment length polymorphism of the PCR-amplified 16S rRNA gene for the identification of Aeromonas spp. Figueras, M.J., Guarro, J. and Martínez-Murcia, A.J. 2000 J. Clin. Microbiol. 38, 2023-2025.

40. 16S ribosomal DNA and 16S-23S spacer diversity of Escherichia coli isolates causing human acute pyelonephritis. Antón, A.I., Martinez-Murcia,A.J., Dalet, F. and Rodríguez-Valera, F. 2001. Clinical Microbiology and Infection 7, 1-7

41. Potential virulence and antimicrobial susceptibility of Aeromonas popofii, recovered from freshwater and seawater. Soler, L., Figueras, M.J., Chacón, M., Vila, J., Marco, F., Martínez-Murcia, A.J. and Guarro, J.2002 FEMS Immunol. Med. Microbiol. Letts. 32, 243-247

42. Comparison of three molecular methods for typing Aeromonas popoffii isolates. Soler L., Figueras M.J., Chacón M.R., Guarro J. & Martinez-Murcia A.J. (2003). Antonie van Leeuwenhoek. 83, 341-349

43. Phylogenetic analysis of the genus Aeromonas based on gyrB gene sequences. Yánez, M.A., Catalán, V., Apráiz, D., Figueras, M.J. and Martínez-Murcia A.J. 2003 Int. J. System. Evol. Microbiol. 53, 875-883.

44. Phylogenetic analysis of the genus Aeromonas based on two housekeeping genes. L. Soler3, M.A. Yáñez2,4, M.R. Chacon3, M.G. Aguilera-Arreola3,5, V. Catalan4, M.J. Figueras3, and A. J.Martinez-Murcia1,2 . 2004. Int. J. System. Evol. Microbiol. 54, 1511-1519

45. Resistance to & -lactam antibiotics in Aeromonas hydrophila isolated from rainbow trout (Oncorhynchus mykiss). Saavedra , Guedes-Novais, Alves, Rema, Tacão, Correia, and Martínez-Murcia. 2004. International Microbiology 7, 207-211.

46. First record of the rare species Aeromonas culicicola from a drinking water supply. Figueras, M.J., Suarez-Franquet, A., Chacón, M.R., Soler, L., Navarro, M., Alejandre, C., Grasa, B., Martínez-Murcia, A.J., and Guarro, J. 2005 Appl. Environ. Microbiol. 71, 538-541.

47. Phenotypifont-size: genotypic and phylogenetic discrepancies to differentiate Aeromonas salmonicida and Aeromonas bestiarum from each other. A.J. Martínez-Murcia* 1, L. Soler2, M.J. Saavedra1,3, M.R. Chacón, J. Guarro2, E. Stackebrandt4, and M.J. Figueras2. 2005 International Microbiology 8, 259-270

48. Microbiota associated to Posidonia oceanica in Western Mediterranean sea. Ester Marco-Noales*,1, Mónica Ordax1, Armando Delgado1, María Milagros López1, María José Saavedra2, Antonio Martínez-Murcia2, Neus Garcias3, Núria Marbà3, Carlos M. Duarte3 2006 . Modern Multidisciplinary Applied Microbiology. Exploiting Microbes and Their Interactions. Mendez-Vilas, Antonio (ed.) 1ª Edition , páginas 114-119. ©2006 Wiley-VCH Verlag GmbH & Co. KGaA

49. Genotyping of isolates included in the description of a novel species should be mandatory. Figueras, M. J., Alperi, A., Guarro J. & Martinez-Murcia A. J. (2006). International Journal of Systematic and Evolutionary Microbiology, 56, 1183-1184

50. Updated phylogeny of the genus Aeromonas M.J. Saavedra1, 2, M.J. Figueras3, and A.J. Martínez-Murcia1* (2006) International Journal of Systematic and Evolutionary Microbiology, 56, 2481-2487.

51. Phylogenetic identification of new Aeromonas allosaccharophila strains isolated from pigs, a species not detected since first description. Saavedra, M. J., Perea, V., Fontes, M. C., Martins, C. & Martínez-Murcia, A. J. (2007). Antonie van Leeuwenhoek, 91, 159-167.

52. The recently proposed species Aeromonas sharmana sp. nov., isolate GPTSA-6, is not a member of the genus Aeromonas. A.J. Martínez-Murcia1*, M.J. Figueras3, M.J. Saavedra1, 2 and E. Stackebrandt (2007) International Microbiology, 10, 61-64.

53. Controversial data on the association of Aeromonas with diarrhoea in a recent Hong Kong study. M. J. Figueras, A. J. Horneman, A.J. Martinez-Murcia and A.J. Guarro (2007) Journal of Medical Microbiology, 56, 996-998.

54. Aeromonas tecta sp. nov., isolated from clinical and environmental sources. Antonella Demarta,1 Mara Küpfer,1 Philippe Riegel2, Colette Harf-Monteil,2 Mauro Tonolla1, Raffaele Peduzzi1, M.J. Saavedra3, A.J. Martínez-Murcia (2008) Syst. Appl. Microbiol. 31, 278–286

55. Aeromonas aquariorum species nova, isolated from aquaria of ornamental fish. A. J. Martınez-Murcia, M. J. Saavedra, V. R. Mota, T. Maier, E. Stackebrandt and S. Cousin (2008) International Journal of Systematic and Evolutionary Microbiology, 58, 1169–1175

56. Analysis of 16S rRNA gene mutations in a subset of Aeromonas strains and their impact in species delineation (2008) Anabel Alperi, Maria J. Figueras, Isabel Inza, Antonio J. Martínez-Murcia International Microbiology, 11:185-194

57. Phylogenetic evidence indicated that Aeromonas hydrophila subsp. dhakensis Huys et al. 2002 is a synonym of Aeromonas aquariorum sp. nov. Martínez-Murcia et al. 2008. Antonio Martínez-Murcia , Arturo Monera, Anabel Alperi, Maria-Jose Figueras, Maria-Jose Saavedra (2009) Current Microbiology 58: 76-80.

58. Cultivar identification using 18S–28S rDNA intergenic spacer-RFLP in pomegranate (Punica granatum L.) P. Melgarejo J.J. Martínez, Fca. Hernández a, R. Martínez,*, P. Legua, R. Oncina, A.J. Martínez-Murcia (2009) Scientia Horticulturae 120: 500–503

59. Clinical Relevance of the Recently Described Species Aeromonas aquariorum_ M. Jose Figueras,* Anabel Alperi, M. Jose Saavedra, Wen-Chien Ko, Nieves Gonzalo, Maria Navarro, and Antonio J. Martínez-Murcia (2009) Journal of Clinical Microbiology 47: 3742-3746

60. Phylogenetic identification of Aeromonas simiae from a pig, first isolate since species description Maria Conceicao Fontes, Maria Jose Saavedra, Arturo Monera, Conceicao Martins, Antonio Martınez-Murcia (2010) Veterinary Microbiology 142: 313-316.

61. Aeromonas fluvialis sp. nov., isolated from a Spanish river. Anabel Alperi, Antonio J. Martínez-Murcia, Arturo Monera, Maria J. Saavedra and Maria J. Figueras Int J Syst Evol Microbiol (2010); 60, 72–77

62. Aeromonas taiwanensis sp. nov. and Aeromonas sanarellii sp. nov., two new clinical species from Taiwan Anabel Alperi, Antonio J. Martínez-Murcia, Wen-Chien Ko, Arturo Monera, Maria J. Saavedra and Maria J. Figueras Int J Syst Evol Microbiol (2010) 60, 2048 - 2055.

63. Aeromonas rivuli sp. nov., isolated from the upstream region of a karst water rivulet in Germany . Maria J. Figueras Anabel Alperi, Roxana Beaz-Hidalgo, Erko Stackebrandt, E. Brambilla, Arturo Monera, and Antonio J. Martínez-Murcia Int J Syst Evol Microbiol (2011) 61, 242-248.

64. Multilocus phylogenetic analysis of the genus Aeromonas. Martinez-Murcia, A. J., Monera, A., Saavedra, M. J., Oncina, R., Lopez-Alvarez, M., Lara, E. and Figueras M. J. (2011) Syst Appl Microbiol 34; 189–199

65. Phylogenetic identification of Aeromonas from pigs slaughtered for consumption in slaughterhouses at the North of Portugal M.C. Fontes, M.J. Saavedra, C. Martins and A.J. Martínez-Murcia. (2011) International Journal of Food Microbiology 146: 118–122

66. Recommendations for a new bacterial species description based on analyses of the unrelated genera Aeromonas and Arcobacter. 2011. María José Figueras, Roxana Beaz-Hidalgo, Luis Collado and Antonio Martínez-Murcia. The Bulletin of BISMiS (2011), Volume 2, part 1, pp. 1–16. (Published by Bergey’s International Society for Microbial Systematics)

67. Antibacterial effects of glucosinolate derived hydrolysis products against enterobacteria and enterococci isolated from pig ileum segments. Saavedra, MJ., Dias, C., Martinez-Murcia, A., Bennett, R., Aires, A., Rosa, E.(2012). Foodborne Pathogens and Disease. April 2012, Vol. 9, No. 4: 338-345.

69.. Aeromonas australiensis  sp. nov., isolated from irrigation water in Western Australia. Max Aravena-Román, Roxana Beaz-Hidalgo, Timothy J.J. Inglis,  T. V. Riley, A. J. Martínez-Murcia, Barbara J. Chang,  and Maria Jose Figueras. Int J Syst Evol Microbiol 2013 Jun;63 (Pt 6):2270-6

70. Aeromonas cavernicolasp. nov., isolated from fresh water of a brook in a cavern. A. J. Martínez-Murcia, R. Beaz-Hidalgo, Pavel Svec, M. Jose Saavedra, M. J. Figueras, and Ivo Sedlacek Curr Microbiol 2013 Feb;66(2):197-204

71. Reclassification of Aeromonas hydrophila subsp. dhakensis Huys et al. 2002 and Aeromonas aquariorum Martínez-Murcia et al. 2008 as Aeromonas dhakensis sp. nov. comb nov. and emendation of the species Aeromonas hydrophila. Roxana Beaz-Hidalgo, Antonio Martínez-Murcia & Maria José Figueras Syst Appl Microbiol  2013 May;36(3):171-6.

72.Detection of Legionella spp. in Natural and Man-made Water Systems Using Standard Guidelines Borges A., Simões M., Martínez-Murcia A., Saavedra M. J. Journal of Microbiology Research 2012, 2(4): 95-102

73. Corrigendum to “Reclassification of Aeromonas hydrophila subsp. dhakensis Huys et al. 2002 and Aeromonas aquariorum Martínez-Murcia et al. 2008 as Aeromonas dhakensis sp. nov. comb nov. and emendation of the species Aeromonas hydrophila” [Syst. Appl. Microbiol. 36 (2013) 171–176] Roxana Beaz-Hidalgo, Antonio Martínez-Murcia, María José Figueras Systematic and Applied Microbiology, Volume 37, Issue 7, October 2014, Page 543

74. Aeromonas lusitana sp. nov., isolated from untreated water and vegetables A. J. Martínez-Murcia, A. Navarro, R. Beaz-Hidalgo, Maria João Carvalho, Max Aravena-Román,  Antonio Correia, M. J. Figueras, and M. Jose Saavedra Current  Microbiol (2016) 72:795–803

75. Arcobacter ebronensis sp. nov. and Arcobacter aquimarinus sp. nov., two new species isolated from marine environment Arturo Levican; Sara Rubio-Arcos; Antonio Martinez-Murcia; Luis Collado; Maria Jose Figueras Systematic and Applied Microbiology - 2015, 38 (1) Pages 30–35

76. Purification and quantification of circulating cell-free DNA from body fluids with DANAGENE Circulating System applied to Liquid Biopsy David Navarro, A. Navarro, A. Martinez-Murcia, and Adriana Lasa BioTechniques, Vol. 60, No. 4, April 2016, p. 212

77. Multilocus phylogenetic analysis of the genus LegionellaMartinez-Murcia, A. J. and co-workers in preparation

78. Multilocus phylogenetic analysis of the genus ArcobacterMartinez-Murcia, A. J. and co-workers in preparation

79. Multilocus phylogenetic analysis of the genus ListeriaMartinez-Murcia, A. J. and co-workers in preparation

 

Research of Veterinary Diseases